Human Cytokine Array G3
Update：2014/7/23 10:34:46 View：3701
- Type AAH-CYT-G3
- Description
Raybiotech Products
Introduction
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 μl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
|
Scroll over each target protein for more information |
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|
ENA-78
(CXCL5) |
GCSF
|
GM-CSF
|
GRO alpha/beta/gamma
|
GRO alpha
(CXCL1) |
|
I-309
(TCA-3/CCL1) |
IL-1 alpha
(IL-1 F1) |
IL-1 beta
(IL-1 F2) |
IL-2
|
IL-3
|
|
IL-4
|
IL-5
|
IL-6
|
IL-7
|
IL-8
(CXCL8) |
|
IL-10
|
IL-12 p40/p70
|
IL-13
|
IL-15
|
IFN-gamma
|
|
MCP-1
(CCL2) |
MCP-2
(CCL8) |
MCP-3
(MARC/CCL7) |
M-CSF
|
MDC
(CCL22) |
|
MIG
(CXCL9) |
MIP-1 delta
(CCL15) |
RANTES
(CCL5) |
SCF
|
SDF-1 alpha
(CXCL12 alpha) |
|
TARC
(CCL17) |
TGF beta 1
|
TNF alpha
|
TNF beta
(TNFSF1B) |
EGF
|
|
IGF-1
|
Angiogenin
|
Oncostatin M
|
Thrombopoietin
(TPO) |
VEGF-A
|
|
PDGF-BB
|
Leptin
|
|||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array G3 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
42
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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