Human Cytokine Array C1
Update：2014/7/21 12:24:53 View：2944
- Type AAH-CYT-1
- Description
Raybiotech Products
Introduction
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
|
Scroll over each target protein for more information |
||||
|
GCSF
|
GM-CSF
|
GRO alpha/beta/gamma
|
GRO alpha
(CXCL1) |
IL-1 alpha
(IL-1 F1) |
|
IL-2
|
IL-3
|
IL-5
|
IL-6
|
IL-7
|
|
IL-8
(CXCL8) |
IL-10
|
IL-13
|
IL-15
|
IFN-gamma
|
|
MCP-1
(CCL2) |
MCP-2
(CCL8) |
MCP-3
(MARC/CCL7) |
MIG
(CXCL9) |
RANTES
(CCL5) |
|
TGF beta 1
|
TNF alpha
|
TNF beta
(TNFSF1B) |
||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It? or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Number of Targets Detected
23
Usage
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
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